辛伐他汀依赖p53通路对K562细胞G_1期调节的分子机制

The molecular mechanism of regulation of G_1 phase in K562 cells treated with simvastatin in a p53 pathway-dependent manner

目的探讨辛伐他汀体外处理后的K562细胞p53通路和K562细胞G1期的分子水平的变化,以说明辛伐他汀是否依赖p53通路参与K562细胞细胞周期G0/G1期停滞的调控和抑制K562细胞增殖。方法体外培养和用辛伐他汀处理K562细胞,用流式细胞术检测辛伐他汀作用后细胞周期和凋亡率的变化,用RT-PCR检测K562细胞p53通路和细胞周期G1期相关基因表达的变化。结果辛伐他汀能使K562细胞停滞在G0/G1期,明显诱导K562细胞凋亡,大多数p53通路基因和细胞周期相关基因的变化出现差异表达。结论辛伐他汀可能通过作用于参与细胞增殖和凋亡的p53通路,抑制K562细胞增殖并诱导细胞凋亡。

Objective To investigate the changes of p53 pathway and G1 phase in K562 cells after being treated with simvastatin in molecular levels, so as to illustrate whether simvastatin is involved in the regulation of G0/G1 arrest and inhibits the proliferation of K562 cells in a p53 pathway dependent-manner. Methods K562 ceils were cultured and treated with simvastatin in vitrol.The apoptosis rate and cell cycle of K562 cells were measured by flow cytometry（FCM） .The expression of p53 pathway and cell cycle were detected by RT-PCR at transcriptional level. Results We shown that G0/G1 arrest of cell cycle and significant apoptosis rate of K562 cells are induced by simvastatin. The most cell cycle related genes and genes of p53 pathway induced were expressed differentially in K562 cells. Conclusion Simvastatin can inhibit the proliferation of K562 cells and induce the apoptosis of K562 cells,depending on p53 pathway which is involved in cell proliferation and apoptosis.