肌瘤清胶囊对子宫肌瘤模型大鼠血清NOS、TNF-α和IL-2水平的影响

Effects of Jiliuqing Capsules on Cytokines in Rats with Hysteromyoma

目的:本研究主要观察肌瘤清胶囊对子宫肌瘤大鼠血清NOS活性及TNF-α、IL-2水平的影响,探讨肌瘤清胶囊治疗子宫肌瘤的可能机制。方法:采用雌激素、孕激素共同肌注的方法建立大鼠子宫肌瘤模型,灌胃给予肌瘤清胶囊11周,检测血清NOS活性和IL-2、TNF-α水平。结果:模型组大鼠血清NOS活性和TNF-α水平较正常组显著升高(P<0.01),IL-2水平显著降低(P<0.01);而肌瘤清胶囊能明显降低血清NOS活性和TNF-α水平(P<0.05或P<0.01),升高血清IL-2水平(P<0.05或P<0.01)。结论:肌瘤清胶囊能明显降低血清NOS活性和TNF-α水平,升高IL-2水平,提示肌瘤清胶囊抑制子宫肌瘤作用可能与调节内分泌、提高机体免疫功能有关。

Background ＆ Objective： Recent studies indicated that nitric oxide synthase （NOS） pathway was involved in angiogenesis around tumor tissues and that tumor necrosis factor-α （TNF-α） and interleukin-2 （IL-2） were concerned with the oncogenesis and development of hysteromyoma. In the present study, the effect of Jiliuqing capsules on serum activity of NOS and concentrations of TNF-α and IL-2 in rats with hysteromyoma was investigated to elucidate the possible mechanisms implicated in the anti-tumor effect of Jiliuqing capsules. Methods： Hysteromyoma in rats was established by intramuscular injection of estrogen and progesterone. Following treatment with Jiliuqing capsules for 11 weeks, serum activity of NOS and concentrations of TNF-α and IL-2 were determined. Results： Compared with those in control group, serum activity of NOS and TNF-α concentration in model group were increased significantly, while serum content of IL-2 was decreased markedly. In contrast, treatment with Jiliuqing capsules resulted in an evident decrease in NOS activity and serum level of TNF-α, together with a markedly increased serum concentration of IL-2. Conclusion： Jiliuqing capsules can significantly decrease serum activity of NOS and TNF-α content and increase serum level of IL-2, suggesting that the anti-tumor effect of Jiliuqing capsules on hysteromyoma might be related, at least partly, to the adjustment of endocrine and enhancement of immune function.