MATER蛋白在人类卵母细胞及着床前胚胎中的表达

Expression of MATER Protein in human oocytes and preimplantation embryos

目的探讨M ater在人卵发生和胚胎早期发育中的表达情况。方法收集体外受精助孕过程中未成熟的卵母细胞及不适合移植及冷冻保存的胚胎,采用免疫荧光联合激光共聚焦显微镜技术检测人类卵母细胞及着床前胚胎MATER蛋白的表达。结果16个未成熟卵母细胞15个MATER蛋白表达阳性;58个2-8细胞期人类早期胚胎中40个MATER蛋白检测阳性;5个囊胚和4个孵化出囊胚内细胞团均为阴性,滋养层细胞为弱阳性。结论MATER蛋白在人类卵母细胞中存在,并随着早期胚胎的发育其表达阳性率逐渐降低,至囊胚期只有滋养层细胞少量表达,这符合母性效应基因在哺乳动物中的表达模式。

Objective： To investigate the function and significance of MATER protein in human oocytes and preimplantation embryos. Methods： Human immature oocytes and preimplantation embryos which were surplus to requirement for clinical treatments were collected. Immunofluorescence staining was used to detect MATER protein in varies stages of human oocytes and preimplantation embryos. Results： By confocal microscopy , MATER was stained positive in 15/16 oocytes and 40/58 2 -cell to 8 -cell human embryos; In five blastocysts and four hatched blastocysts, MATER protein was absent in the inner cell mass , only the trophectoderm shows weak positive. Conclusions ： MATER protein expressed in human oocytes and decreases along with the stages of embryo development. In blastocysts it only shows little expression in the trophectoderm. This expression patterns are consistent with a maternal effect function conserved in mammalians .