摘要
龙牙蕉吸芽茎尖经MS+6-BA 5.0 mg/L+AD 20 mg/L培养基诱导出不定芽后,在MS+BA 4 mg/L+NAA 0.1 mg/L培养基上继代培养对不定芽的分化较好,平均分化倍数为2.6倍以上,无根小苗在1/2 MS+NAA 0.5 mg/L+AC 1 g/L培养基上进行生根培养,1周后长出新根。组培小苗移栽假植成活率94%,大田栽培表现正常。
Adventitious buds were induced from shoot-tip explants of Musa spp Longyajiao in MS＋6-BA 5.0 mg/L＋AD 20 mg/L. The multiplication medium with MS＋BA 4 mg/L＋NAA 0. 1 mg/L was better for the buds growth and the average multiplication rate was over 2.6 times. Plantlets were cultured on 1/2 MS＋ NAA 0. 5 mg/L＋AC 1 g/L, for rooting culture, after 7 days the roots of the plantlets were developed. The regenerated plantlets were transplanted to soil, and the survival rat of transplanting reached 94%. The plants grew well in the field.
出处
《南方园艺》
2009年第2期10-11,共2页
Southern Horticulture
关键词
龙牙蕉
组织培养
细胞分裂素
Musa spp Longyajiao
Tissue culture
Cytokinin
