一种定量测定Pb^(2+)的新型荧光探针的合成及性能

Synthesis and Properties of a Novel Fluorescent Probe for Quantitative Measurement of Pb^(2+)

以氟化硼络合二吡咯甲川类(BODIPY)染料为荧光团,二(2-羟基乙基)亚胺为识别体合成了一种新的对Pb2+具有高选择性的Pb2+荧光探针。在HEPES缓冲溶液(pH7.4)中,当Pb2+浓度达到1.0×10-5mol/L时肉眼可观察到探针荧光强度明显增强;当Pb2+浓度达到3.0×10-4mol/L时,探针荧光强度增大20倍以上,且识别作用不受其它金属离子(Na+、Mg2+、Ca2+、Cu2+、Fe2+、Hg2+、Ag+、Cd2+、Cr3+、Fe3+)干扰。Pb2+浓度在0.2×10-5～5.0×10-5mol/L范围内,探针荧光强度与Pb2+浓度有较好的线性关系,相关系数为0.998,检出限为0.2μmol/L。

Taking BODIPY（4, 4-difluoro-4-bora-3a, 4a-diaza-s-indacene） dye as a fluorophore, dihydroxydiethylamine as a receptor, a novel fluorescent probe for Pb^2＋ detection was synthesized with good selectivity. In HEPES（pH = 7.4） buffer, when the concentration of Pb^2＋ reached 1.0 × 10^-5 mol/L, the fluorescent intensity of the probe was enhanced obviously even to be observed with naked eyes ; when the concentration reached 3.0 × 10^-4 mol/L, the fluorescent intensity enhanced over 20- fold. In addition, other metal ions （Na^2＋, Mg^2＋, Ca^2＋, Cu^2＋, Fe^2＋, Hg^2＋, Ag^＋, Cd^2＋, Cr^3＋ , Fe^3＋ ） didn＇t interfere with the probe＇s recognition for Pb^2＋ When the concentration of Pb2＋ was in the range of 0.2 × 10^-5 -5.0 × 10^-5 mol/L, there was a good linearity between the fluorescence intensity of the probe and the concentration of Pb^2＋ with a correlation coefficient of 0. 998, and the detection limit was 0. 2 μmol/L.