对巯基苯硼酸/纳米金修饰玻碳电极用于葡萄糖的识别

Recognition of Glucose at Glassy Carbon Electrode Modified with 4-Mercaptophenylboronic Acid/Nano-gold Films

采用自组装技术,将对巯基苯硼酸(MPBA)自组装于带正电荷纳米金(nano-gold,NG)修饰的玻碳电极(GCE)表面,从而制得用于识别葡萄糖的无酶传感器(MPBA/NG/GCE)。通过交流阻抗技术和循环伏安法考察了MPBA/NG/GCE修饰电极的表面电化学特性,同时研究了葡萄糖在该修饰电极上的电化学行为,讨论了利用该修饰电极测定葡萄糖的最佳条件。结果表明:在优化的条件下,氧化峰电流与葡萄糖浓度在1.0～150.0mmol/L范围内呈良好的线性关系,其回归方程为ΔIp(μA)=3.37+0.342c(mmol/L),相关系数为r=0.999,检出限为3.8×10-5mol/L(S/N=3),可用于葡萄糖分子的电化学识别。

A 4-mercaptophenylboronic acid/nano-gold film modified glassy carbon electrode（ MPBA/ NG/GCE） was fabricated based on the self-assembly of MPBA on the surface of glassy carbon electrode modified with nano-gold particles and its surface electrochemical characteristic was evaluated by cyclic vohammetry and electrochemical impedance spectroscopy. The electrochemical behavior of glucose on the modified electrode was studied and the optimum conditions for the determination of glucose using this modified electrode were discussed. Results showed that the presence of gold nanoparticles could enhance the electro-activity of glucose at the modified electrode. The current response of MPBA/NG/GCE increased linearly with the glucose concentration in the range of 1.0 - 150. 0 mmol/L with a detection limit of 3.8 × 10^-5 mol/L（S/N =3）. The linear regression equation is A/p（ixA） = 3.37 ＋0. 342c（mmol/L） with a correlation coefficient of 0. 999. This method can be applied to the recognition of glucose.