检测65KD热休克蛋白抗体对Ⅰ型糖尿病诊断的意义

The diagnostic significance of detecting 65KD heat shock protein antibody for type Ⅰdiabetes mellitus

利用卡介苗（BCG）65KD热休克蛋白作为抗原，ELISA法检测Ⅰ型糖尿病患者血清中64KD抗体。对30例Ⅰ型糖尿病、30例Ⅱ型糖尿病、5例系统性红斑狼疮（SLE）、8例甲状腺机能亢进（甲亢）的患者及44例健康人血清进行检测。结果表明，64KD抗体在Ⅰ型糖尿病中的检出率为83．3％，在Ⅱ型糖尿病中的检出率为13．3％，在SLE及甲亢中均为阴性，健康人仅1例阳性。此方法的批内变异系数为4．57％～8．62％，批间变异系数为5．57％～12．7％。诊断特异性为94．6％，诊断敏感性为83．3％，诊断指数为177．9％。

BCG 65KD heat shock protein was taken as antigen to detect serum 64 KD antibody in type Ⅰdiabetes mellitus(DM) by ELISA. The results of 30 cases with type Ⅰ DM, 30 cases with type Ⅱ DM, 5 cases with systemic lupus erythematosus(SLE), 8 cases hyperthyroidism and 44 healthy individuals showed 64KD antibody was positive in 83.3% of type Ⅰ DM, 13.3% of type Ⅱ DM, but negative in all SLE and hyperthyroid patients, and only one positive in the healthy individuals. CV of this method is 4.57%～8.62% within group, and 5.57 %～12.7% among groups. Diagnostic specificity is 94.6%. Diagnostic sensitivity is 83.3%. Diagnostic index in 177.9%.