往复运动式间歇低氧箱的研制

Development of Reciprocating Sports Intermittent Hypoxia Chamber

目的:研究制造外置往复运动式间歇低氧箱,用于建立间歇低氧大鼠动物模型。方法:设立两个动物舱(280mm×200mm×150mm)和一个低氧舱(800mm×800mm×600mm),动物舱中各放入4只SD实验大鼠,低氧舱内持续充入高纯氮以保持舱内低氧环境,伺服电动机驱动低氧舱在两个动物舱之间做往复运动,使两个动物舱交替进出低氧舱,动物舱内实验动物即交替获得低氧环境。设定低氧舱内目标氧体积分数为(10±0.5)%,低氧舱运动周期为180s,其单向运动时间为4s,低氧舱滞留时间86s(即实验动物间歇低氧时间为90s),每天运行8h。分别测定动物舱处于低氧舱内外时的大鼠血气值。结果:低氧箱运行安全可靠;动物舱位于低氧舱内时实验大鼠的最低氧分压为(4.766±0.536)kPa(即(35.75±4.02)mmHg),最低氧饱和度为0.69±0.08,达到了中重度阻塞性睡眠呼吸综合征(OSAS)患者诊断标准。结论:自行研制的外置往复运动式间歇低氧箱可用于建立慢性间歇低氧大鼠动物模型。

Objective To research and develop the model of external reciprocating sports intermittent hypoxia box for rats.Methods Two animal cabins （28 cm×20 cm×15 cm） and one hypoxia chamber （80 cm×80 cm×60 cm） which covered each cabin in turn were developed. 4 male SD rats were placed in each animal cabin. The hypoxia chamber in which low 02 concentration was maintained with filling high-pure Nitrogen continuously, moved to and fro between two animal cabins, and driven by a servomotors. Two animal cabins were covered in turn by the hypoxia chamber and get the same low 02 concentration as in the hypoxia chamber. The goal volume fraction of oxygen in hypoxia chamber was （10±0.5）% and the time of unilateral movement from one animal cabin to another was 4 s, the cycle time of intermittent hypoxia was 180 s, the retention time was 86 s （including the hypoxia and normoxia time was 90 s respectively）. The hypoxia chamber runs 8 h per day. The numerical value on artery blood gas of rats in which hypoxia chamber inside and outside was measured Results The reciprocating sports intermittent hypoxia chamber can be operated safely and reliably. The lowest PaO2 is （4.766± 0.536） kPa（or （35.75±4.02） mmHg） and the lowest SaO2 is 0.69 ±0.08 when the animal cabin in hypoxia chamber. Conclusion The chronic intermittent hypoxia model for rats is established by self-developed external reciprocating sports intermittent hypoxia chamber. [Chinese Medical Equipment Journal, 2009,30 （3） ： 21-22]