鳡鱼群体遗传多样性的AFLP分析

Analysis of Genetic Diversity of Elopichthys bambusa by AFLP Markers

利用AFLP分子标记技术对长江下游苏州段野生和野生F1代人工养殖的2个鳡鱼(Elopichthys bambusa)群体遗传多样性进行分析研究.6对选择性引物在2个群体47个个体中,共扩增出313个位点,多态位点47个.鳡鱼野生和野生F1代人工养殖2个群体的多态位点比率和Shannon’s指数分别为13.42%、8.31%,0.0544、0.0320;前者遗传多样性较后者略高.基因分化系数Gst和Shannon’s指数分析均显示2个鳡鱼群体之间出现一定遗传分化.鳡鱼UPGMA系统树有分支出现且依据群体分别聚类,表现出一定的遗传趋异.结果分析表明,鳡鱼群体的遗传多样性相对贫乏;野生F1代人工养殖群体尚未形成自己独立的遗传结构,但2个群体间已经产生了一定的遗传分化,经过较多世代的人工繁育有可能形成自己独立而稳定的遗传结构.

Genetic diversity of a wild populations and a cultured population of Elopichthys bambusa was investigated using amplified fragment length polymorphism （AFLP）. The wild population was collected from Suzhou area of Yangtze River and the cultured population was hatchery-reared offspring of wild caught individuals from the same area. Six EeoRI and MseI primer combinations produced 47 polymorphie markers out of the total of 313 bands amplified from 47 total individ- uals. The proportions of polymorphic loci of the wild population and the cultured population were 13.42% and 8. 31%, respectively. These Shannon＇ s information index and average heterozygosity were 0. 054 and 0. 032, 0. 341 and 0.199, respectively. The results showed that there was a little differentiation in the genetic diversity index of these two populations. Gst value and Shannon＇ s information index indicated that there was some genetic differentiation between populations. The UPGMA tree based on AFLP data supported this result. In this paper, the results of AFLP analysis indicated that two populations of E. bambusa presented lower genetic diversity. Moreover, some genetic differentiation were showed between two populations, and the cultured population will maybe form stable owe genetic structure by multi-generation artificial breeding.