PCR技术对肺炎支原体DNA的检测

Detection of Mycoplasma pneumonia DNA by PCR Technique

作者首次在国内利用自己的条件完成了肺炎支原体从培养基制备、实验室培养、菌种检定、引物设计、ＤＮＡ提取．到ＰＣＲ扩增条件的摸索、产物检测等一整套技术，与非肺炎支原体、部分相关细菌进行了鉴别，并同时进行了临床样品和模拟样品的实验，结果表明该方法的特异性和灵敏性都是好的．

A set of techniques for the media preparation,the laboratory cultures,the seed identification,the primers design,the DNA extract,the PCR amplification and the PCR products detection of mycoplasma pnuemonia have been done,the differential experiments of both,mycoplasma pnuemonia and non mycoplasma pnuemonia,mycoplasma pnuemonia and some bacteria have been done too.Meanwhile,some clinical samples be detected and some simulated tests be done,the results are satisfactory.