摘要
为获得猪源弓形虫野生虫株,对细胞培养过程中的培养基种类、血清用量及培养佐剂等条件进行优化,选择最佳分离条件。对临床血清学检测呈阳性猪场的19份血液样品进行弓形虫虫株的培养检测,共获得野生虫株10株。运用PCR方法进行初步鉴定,证实分离虫株为弓形虫野生虫株。结果表明,细胞培养方法是一种分离弓形虫虫株较为理想的方法。
To obtain the Toxoplasma gondii field strains from pigs, the kinds of media, serumconcentration and the usage of supernatant of RH strain culture （SRC） were first optimized. Total of 19 blood samples of which the T. gondii antibody are positive were cultured with PK - 15 cells and 10 strains of T. gondii were isolated. The results of PCR and ELISA further confirmed that all of the isolates are field strains. Our data demonstrate that the method of cell culture is an effec- tive way to isolate T. gondii field strains from pigs.
出处
《河南农业科学》
CSCD
北大核心
2009年第3期103-105,共3页
Journal of Henan Agricultural Sciences
基金
教育部重点科研项目(105120)
农业公益性行业科研专项经费项目(200803017)
关键词
弓形虫
虫株
分离
细胞培养
Toxoplasma gondii Strain Isolation
Cell culture
