摘要
以原位ＰＣＲ方法在培养的ＨＩＣＭＡ细胞中进行了Ｙ染色体上单拷贝ＳＲＹ基因的原位扩增，并使用ＰＣＲ方法制备的地高辛标记探针原位检测了所扩增的片段．比较研究表明，原位ＰＣＲ法较之直接的原位杂交法。
The single copy SRY gene was amplified by in situ PCR in cultured HICMA cells and the amplified products were detected with digoxigenin labeled probes generated using the PCR. Comparative studies indicated that in situ PCR is more than 5 times sensitive than in situ hybridization.
出处
《武汉大学学报（自然科学版）》
CSCD
1998年第2期225-228,共4页
Journal of Wuhan University(Natural Science Edition)
基金
国家自然科学基金
关键词
培养细胞
SRY基因
原位PCR法
染色体
in situ Polymerase Chain Reaction, in situ Hybridization, cultured cells, SRY gene
