一种鉴定胸腺素高活性小分子组分的灵敏简便方法

A SENSITIVE AND CONVENIENT METHOD TO DETERMINATE HIGH ACTIVE THYMOSIN COMPONENT WITH LOW MOLECULAR WEIGHT

用笔者稍加改进的梯度ＰＡＧＥ和ＳＤＳ－ＰＡＧＥ尿素系统，分别测定自制的胸腺素Ｔ１溶液，观察到一条５．３ｋｕ的电泳谱带．Ｔ１溶液用Ｅ－玫瑰花结法测定时，生物活力较高。

The purified thymosin T 1 solution was determined by modified gradient PAGE and urea SDS PAGE respectively. The 5.3 ku band was observed. The bioactivity of T 1 solution was 181 times higher than that of crude extractsby E rosette assay.