大豆干旱和低温cDNA文库的构建与检测

Construction and Identification of cDNA Libraries of Soybean Treated by Drought and Low Temperature

以栽培大豆（Glycine max）吉林35为材料，在幼苗期进行干旱、低温处理，分离mRNA经逆转录合成cDNA后，插入到融合表达载体中，分别构建了干旱和低温两个融合表达文库。检测表明，两个初始文库的滴度分别为2．75×10^6pfu·mL^-1和5．52×10^5pfu·mL^-1插入的cDNA片断长度在0．5～2．0kb。此文库的构建为今后分离与干旱、低温相关基因奠定了基础。

Two kinds of mRNA were isolated from cultivated soybean variety Jilin 35 pre-treated with drought and low tem- perature at seedling stage, anti-transcripted into eDNA, to construct two relevant fusion expression libraries with insertion of these eDNA collection. The primary analysis showed that the titer of two initial libraries were 2.75×10^6 pfu·mL^-1（drought） and 5.52×10^5pfu·mL^-1（low temperature）. The size of mostly insertion was between 0. 5and 2. 0 kb. The construction of these two libraries will be helpful for the related gene isolation in the future.