人工流产与复发性自然流产蜕膜淋巴细胞亚群FAS表达的研究

Analysis on the expression of FAS antigen in lymphocyte subsets of decidua in recurrent spontaneous abortion

目的:研究不明原因复发性流产(RSA)患者外周血、蜕膜组织T细胞、NK细胞数量,NK细胞亚群数量及FAS抗原的表达,探讨其在RSA中的作用。方法:选取确定妊娠并难免流产者26例为实验组,25例正常妊娠行人工流产者作为对照组,用流式细胞仪检测两组外周血和蜕膜组织T细胞、NK细胞数量,NK细胞亚群数量及FAS抗原的表达。结果:①对照组外周血T细胞和NK细胞,蜕膜组织T细胞和NK细胞分别为(65.54±11.91)%、(12.26±6.46)%、(5.63±5.10)%、(56.50±19.80)%;实验组分别为(42.41±10.03)%、(12.24±6.40)%,(5.62±4.74)%、(60.14±21.52)%;两组差异无统计学意义(P>0.05)。②对照组外周血CD3-CD56+CD16+和CD3-CD56+CD16-细胞,蜕膜组织CD3-CD56+CD16+和CD3-CD56+CD16-细胞分别为(65.54±11.91)%、(6.06±3.05)%,(2.84±1.59)%、(83.79±6.02)%;实验组分别为(74.93±11.42)%、(5.58±2.72)%,(5.36±4.76)%、(74.12±11.32)%;P值分别是0.014,0.592,0.031,0.001,除外周血CD56+CD16-细胞两组无差异外,其余3组差异有统计学意义。③对照组外周血CD3-CD56+CD16+FAS和蜕膜组织CD3-CD56+CD16-FAS表达分别为(49.15±9.77)%和(4.97±2.32)%;实验组分别为(38.21±9.42)%,(9.59±6.42)%;P值分别是0.001、0.004,差异有统计学意义。结论:FAS/FASL系统可能是外周血和蜕膜组织NK细胞亚群凋亡的主要途径之一。

Objective： To study the expression of FAS antigen and the contents of T lymphocyte, NK lymphocyte, NK lymphocyte subsets in peripheral blood and decidua tissues in women with recurrent spontaneous abortion （RSA） . Methods： 26 pregnancy women with RSA were experimental group, and 25 pregnancy women undergoing induced abortion were control group. The expression of FAS antigen and the contents of T lymphocyte, NK lymphocyte, NK lymphocyte subsets in peripheral blood and decidua tissues were detected by flow cytome- try. Results ： ①The proportions of T lymphocyte and NK lymphocyte in control group were （ 65.54 ±11.91 ） % and （ 12.26± 6.46）. % in peripheral blood, （5.63 ± 5.10） % and （56. 50 ± 19. 80） % in decidua tissues. The proportions of T lymphocyte and NK lymphocyte in ex- perimental group were （42.41±10.03 ） % and （ 12. 24±6/40） % in peripheral blood, （5.62 ±4. 74） % and （60. 14±21.52） % in decidua tissues. There was no significant difference between the two groups. ②The proportions of CD3 - CD56 ＋ CD16 ＋ and CD3 - CD56 ＋ CD16 - in control group were （65.54 ± 11.91 ） % and （6.06 ± 3.05 ） % in peripheral blood, and （2. 84 ± 1. 59） % and （83.79 ± 6. 02） % in decidua tissues. The proportions of CD3 - CD56 ＋ CD16 ＋ and CD3 - CD56 ＋ CD16 - in experimental group were （74.93± 11.42） % and （5.58±2.72）% in peripheral blood, （5.36±4.76）% and （74. 12± 11.32）% in decidua tissues. ③The expression of FAS in CD3 - CD56 ＋CD16 ＋ of peripheral blood and CD3- CD56 ＋ CD16 - of decidua tissue in control group were （49.15±9. 77）% and （4.97± 2. 32）%, （38.21± 9. 42）% and （9. 59 ± 6. 42）% in experimental group, respectively. There was significant difference between the two groups. Conclusion： FAS/FASL system may plays an importment role in the apoptosis of NK subset in peripheral blood and decidua tissues.