洁泽2号对小鼠滴虫性阴道炎预防作用的实验研究

The effect of Jieze NO.2 jel on trichomonas vaginitis in rats

目的:观察中药复方洁泽1号(JZ1)与有效避孕剂量的外用杀精剂壬苯醇醚(N-9)的复合制剂洁泽2号(JZ2)对小鼠滴虫性阴道炎的预防作用。方法:将112只小鼠随机分为JZ2组(高、中、低)、JZ1组(高、中、低)、壬苯醇醚(N-9)组、甲硝唑组、空白凝胶组及模型组。造模前,每只小鼠阴道给予相应的药物凝胶或生理盐水20μl,注药2min后每只小鼠接种滴虫液20μl,在接种后的2、4、7、10、12、14天取小鼠阴道冲洗液涂片镜检及培养,观察小鼠感染情况,并计算各组小鼠不同时间的感染率。结果:JZ2组、N-9组小鼠感染率都为0,与相同时间模型组比较差异有显著性意义(P<0.01);甲硝唑组小鼠感染率很低,前4天仅为10%,之后为0,前10天与模型组比较有显著性差异(P<0.01);JZ1组小鼠滴虫感染率比模型组低,2g.ml-1浓度组在第2天与模型组比较差异有显著性意义(P<0.05);空白凝胶组小鼠在不同时间的滴虫感染率与模型组比较差异均无显著性意义(P>0.05);JZ2组、N-9组、JZ12g.ml-1和1g.ml-1剂量组小鼠滴虫感染率与甲硝唑组比较差异无显著性意义(P>0.05);JZ2与JZ1组比较,0.5g.ml-1组在第2天感染率差异有显著性(P<0.05)。结论:预防性给药后,JZ2高、中、低剂量组和N-9组都能完全预防小鼠滴虫性阴道炎的感染,其作用强于同浓度的JZ1。

Objective： To observe the preventive effect of Jieze NO. 2 jel and nonoxynol - 9 on trichomonas vaginitis in rats. Methods： 112 rats were divided into Jieze NO. 2 group （high titer, median titer and lower titer） , Jieze NO. l group （high titer, median titer and lower titer） , nonoxynol -9 group, Metronidazole group, jel control group and model group. 112 rats were inoculated by 20 μl jel of different drugs or saline before modeling composition, 2 minutes later, all rats were inoculated by 20 μl trophozoites. Vaginal douche fluids were collected to test under microscope and culture in the medium at 2, 4, 7, 10, 12, 14 days after inoculation, infection rates were compared between each group. Results： There was significant difference in infection rate between Jieze NO. 2 group （0） , Jieze NO. 1 group （0） and model group （ P 〈 0.01 ） ; The infection rate of metronidazole group was 10. 0% at 4 days, which was lower than that of control group at 10 days （ P 〈 0.01 ） ; the infection rate of Jieze NO. 1 2 g · ml^-1 group was lower than that in control group at 2 days （ P 〈 0. 05 ） ; there was no significant difference in infection rate between metronidazole group and Jieze NO. 2 group, N - 9 group, 2 g·ml^-1 and 1 g · ml^-1 of Jieze NO. 1 group （ P 〉 0. 05 ） ; there was significant difference in infection rate of 0.5 g · ml^ -1 between Jieze NO. 2 group and Jieze NO. 1 group at 2 days （ P 〈 0. 05 ） . Conclusion ： The high titer group, the median titer group and the lower titer group of Jieze NO. 2 all can prevent the occurrence of trichomonas vaginitis effectively.