卡拉胶低聚糖的辐射防护及其作用机制研究

Radioprotective Effect and its Mechanism of the Carrageenan with Low Molecular Weight

目的探讨卡拉胶低聚糖对受X射线照射小鼠的辐射防护作用及其机制。方法采用X射线一次全身照射小鼠,观察6.0Gy照射小鼠30d存活率,检测外周血指标,脾脏指数,血清和肝脏中抗氧化酶和脂质过氧化物水平。结果卡拉胶低聚糖组小鼠30d存活率比照射对照组显著提高,而且卡拉胶低聚糖能加速小鼠外周血白细胞总数(WBC)、血小板数(PLT)和红细胞数(RBC)的恢复,明显提高脾脏指数,并且可引起谷胱甘肽过氧化物酶(GSH-Px)和超氧化物歧化酶(SOD)活性显著增高,脂质过氧化产物丙二醛(MDA)含量显著下降。结论卡拉胶低聚糖对受照射小鼠有明显的辐射防护作用,其机理可能与其保护造血组织和抗氧化作用有关。

Objective To study the radioprotection effect and its mechanism of the carrageenan with low molecular weight on irradiated mice. Methods The carrageenan with low molecular weight is applied to mice irradiated with 6.0 Gy rays. 30 -day survival rate was observed. The peripheral white blood cell counts, thrombocytes and erythrocytes were meas- ured on 0, 7, 14 and 21 days respectively after irradiation. At various time points after irradiation, the activities of the antioxidant enzymes SOD and GSH - Px were assayed in the blood and liver tissues, and the damage to the cell membrane was studied by measuring lipid peroxides MDA levels. The spleen index was measured on day 21 after irradiation. Results 30 - day survival rate significantly increased in carrageenan with low molecular weight group. The carrageenan with low molecular weight significantly promoted recovery of peripheral white blood cell, thrombocytes and erythrocytes, markedly increased spleen index. The carrageenan with low molecular weight significantly enhanced activities of the GSH - Px and SOD in blood, and decreased the MDA level in liver. Conclusion The carrageenan with low molecular weight has marked radioprotective effect on irradiated mice and the biological effect might be related to the hematopoiesis recovery and antioxidant activation.