摘要
以ATP2B1、HRH1基因作为仔猪腹泻相关的候选基因,在2个品种63头猪的小肠组织中,采用实时荧光定量PCR技术对肠毒素大肠杆菌(ETEC)F4受体不同黏附表型个体进行表达量的比较分析,以验证其基因功能和作为仔猪腹泻相关候选基因的可能性.结果表明:ATP2B1基因在不同黏附表型间的表达量有显著的差异,而HRH1基因在品种间以及黏附表型间表达量差异均不显著.ATP2B1基因可望作为F4ab受体的相关候选基因,而HRH1基因仍需进一步研究.
As candidate genes relevant to diarrhea traits in piglets, the genetic expression ofATP2B1, HRH1 genes were detected in 63 pigs including 2 breeds by mean s of real-time fluorescent quantitative PCR (FQ-PCR) to check their function and the possibility of being related candidate genes in contrast with the differences between the non-adhesived and adhesive. Analysis showed that the ATP2B1 gene's expression produced significant differences between the non-adhesived and adhesive, however, the HRH1 gene did not. According to the FQ-PRC technique, the ATP2B1 gene seemed to be the related candidate genes to piglet's diarrhea, but further study on HRH1 gene needs to be done.
出处
《湖南农业大学学报(自然科学版)》
CAS
CSCD
北大核心
2009年第1期84-87,共4页
Journal of Hunan Agricultural University(Natural Sciences)
基金
国家自然科学青年基金项目(30800607)