高氧致新生大鼠肠黏膜SIgA的变化

Changes in Secretory IgA Induced by Hyperoxia in Intestinal Mucosa of Neonatal Rats

目的研究高氧对新生大鼠肠黏膜分泌性免疫球蛋白A(SIgA)变化的影响。方法建立高氧动物模型,HE染色观察肠道形态学变化,采用放射免疫方法检测高氧对新生大鼠肠黏膜分泌SIgA的影响。结果与空气对照组比较,形态学上高氧组肠道无变化。高氧组新生大鼠回肠黏液SIgA较空气对照组明显升高(P<0.01),但结肠黏液SIgA与空气对照组比较无明显变化(P>0.05)。结论在持续高氧作用下新生大鼠回肠黏膜分泌大量SIgA,这有利于抵御细菌、毒素的侵袭,以保持肠黏膜的完整性。

Objective To study the changes in secretory IgA（SIgA ）induced by hyperoxia in the intestinal mucosa of neonatal rots.Methods The rat model of hyperoxia was established.The histological changes of gut were observed after HE staining.The effect of hyperoxia on SIgA was detected by radioimmunoassay. Results There was no sigrificant difference in histological changes between hyperoxia and control groups.The level of SIgA in ileal mucus in hyperoxia group was significantly higher than that in control group （P 〈 0.01 ）,but no significant difference in the level of SIgA in colonic mucus was found between these 2 groups （P 〉 0.01 ）. Conclusion A lot of SIgA would be secreted by ileum mucosa in neonatal rats exposed continuously to hyperoxia,which could protect the intestinal mucosa against toxins and bacteria and preserve the integrity of intestinal mucosa.