喉鳞状细胞癌中微卫星的异常改变

Microsatellite alternation in laryngeal squamous cell carcinomas

目的:从分子生物学水平探究微卫星的不稳定性(MSI)与杂合性缺失(LOH)在喉鳞状细胞癌发病机制中的意义。方法:选择3号,5号及11号染色体的3个微卫星位点采用PCR和聚丙烯酰胺凝胶电泳-硝酸银染色方法对40例喉鳞状细胞癌患者手术切除的癌组织及癌旁正常组织进行微卫星分析。结果:40例喉鳞状细胞癌中,35例(87.5%)分别有1～3个微卫星位点发生MSI或LOH。微卫星异常改变发生率最高的位点为D5s592,占70%(28/40);其次是D3s1228位点,占52.5%(21/40)。结论:在3p14区域及5q23区域附近的抑癌基因参与致癌机制,D3s1228和D5s592的微卫星改变与喉鳞状细胞癌的临床分期相关。

Objective： To examine the microsatellite instability and loss of heterozygosity in the pathogenic mechanism of laryngeal squamous cell carcinomas. Method：Forty cases squamous cell carcinomas of larynx were analyzed by comparing tumorous tissues and normal tissues around with 3 mierosatellite markers from chromosome 3,5 and 11 ,using PCR and PGE-AgNO3 staining. Result：Among the 40 cases of laryngeal squamous ceil carcino- mas,87.5% （35/40）of samples showed microsatellite instability or loss of heterozygosity in one to three microsat- ellite markers. High frequent microsatellite abnormal occurred at D5S592 ,it was 70% （28/40）. Then the mutation rate of D3s1228 was 52.5 % （21/40）. Conclusion：Our studay revealed that tumor suppressor genes nearby chromo- some 3p14 and 5q23 regions related to the pathogenesis of squamous cell carcinomas of larynx . A correlation be-tween microsatellite alternation and stage of the tumor were found in D3s1228 and DSs592 chromosome regions.