不同采收时期猫豆中左旋多巴的含量比较

A Comparative Study on the Contents of Levedopa in Seed of Stizolobium cochinchinensis(Lour) Tang et Wang in Different Harvesting Time

目的考察与确定猫豆的最佳采收季节。方法采用高效液相色谱法测定不同成熟时期猫豆中左旋多巴的含量。以Phenomenex C18柱（250mm×4．6mm，5μm）为色谱柱，流动相为0．1mol·L^-1冰醋酸溶液：甲醇（95：5）；流速1．0ml·min^-1；检测波长280nm；柱温25℃。结果5种不同成熟时期猫豆中左旋多巴的含量在3．47％～6．55％之间。结论干枯期采收的猫豆左旋多巴含量最高（6．55％），是猫豆的最佳采收时期。

Objective To review and determine the optimal harvesting time of seed of Stizolobium cochinchinensis （Lour） Tang et Wang. Methods Determined their levedopa content with HPLC. A Phenomenex C18 column（250 mm ×4.6 mm, 5 μm） was selected as separation column at 25℃. 0.1 mol · L^-1 acetic acid solution ：methanol（95：5） was used as mobile phase at a flow rate of 1.0ml/min. The detection wavelength was set at 280nm. Results The range of levedopa contents in seeds at different mature periods were 3.47% -6.55%. Conclusion The quality of withered one is the best in those seeds（6.55% ）. Withered period is the optimal harvesting time.