高效液相色谱法测定白花蛇舌草中3，4-二羟基苯甲酸甲酯的含量

Determination of 3,4-dihydroxy Methyl Benzoate in Hedyotis diffusa Willd. by HPLC

目的建立测定白花蛇舌草中3，4-二羟基苯甲酸甲酯含量的高效液相色谱（HPLC）法。方法采用ODS柱（200mm×4．6mm，5μm），以甲醇-0．05％磷酸水溶液（15：85，V/V）为流动相，在254nm波长处检测。3，4-二羟基苯甲酸甲酯浓度在2．5～50μg·ml^-1范围内与峰面积呈良好的线性关系（r=0．9994）；样品的加样回收率为100．4％（n=9）；结果精密度及重复性良好，RSD均不大于2．6％。结论该法简便、准确，可以作为对白花蛇舌草药材进行质量控制的一种定量。

Objective To develop a method for the determination of 3,4 - dihydroxy methyl benzoate in Hedyotis diffusa Willd.. Methods ODS （200 mm × 4.6 mm, 5 μm）column was used with a mobile phase consisted of mixture of methanol -0.05% phosphonic acid （ 15： 85, V/V） by flow rate of 0.8 ml · min^ -1 in this method. UV detector wavelength was 254 nm. Results There was good linear relationship within the range of 2.5 - 50 μg · ml^-1 （r = 0. 999 4）. The average recovery was 100.4% （n = 9）. Both the precision and reproducibility were good with RSD of 2.3% and 2.6% , respectively. Conclusion An accurate, simple quantitative method with good reproducibility to assay 3,4 - dihydroxy methyl benzoate in Hedyotis diffusa Willd. was developed.