高效液相色谱法测定川木通中豆甾醇的含量

Determination of Stigmasterol in Clematis armandii Franch.and Clematis montana Buch.-Ham.by HPLC

目的建立用高效液相色谱（HPLC）法测定川木通中豆甾醇的含量。方法色谱柱为Diamonsil C18柱（150mm×4．6mm，5μm），流动相为甲醇-水（97：3），检测波长为210nm，流速1．0ml·min^-1，进样量5μl，柱温30℃。结果豆甾醇进样量在1．835～18．35μg范围内具有良好的线性关系，r=0．9999，回收率为101．0％～103．6％，RSD为0．7％～2．1％（n=3）。结论该法准确性和重复性好，可用于川木通的质量控制。

Objective To develop an HPLC method for the content determination of stigmasterol in Clematis armandii Franch. and Clematis montana Buch. -Ham. Methods In the system of HPLC, a Diamonsil C18 （4.6 mm × 150 mm ,5 μm）column was used , with methanol -water （97： 3 ） as mobile phase. The flow rate was 1.0 ml · min ^-1. The detection wavelength was set at 210nm and the column temperature was 30℃. Results Stigmasterol curve was linear over the range of 1. 835 - 18.35 μg （ r = 0. 999 9）. The average recovery of the method was 101.0% - 103.6% （RSD =0.7% -2.1% ,n =3）. Conclusion The proposed method is convenient, economical and accurate with good reproducibility, and can be used to evaluate the quality of Clematis armandii Franch. and Clematis montana Buch. -Ham.