脑力智宝对不完全脑缺血损伤大鼠的保护作用

Protective Effect of Naolizhibao on Incomplete Cerebral Ischemia in Rats

目的研究脑力智宝对不完全脑缺血损伤大鼠的保护作用。方法采用结扎双侧颈总动脉制作不完全脑缺血损伤大鼠模型,连续给药30 d后,进行血液流变学、脑组织丙二醛(MDA)与一氧化氮(NO)的含量、超氧化物歧化酶(SOD)活力的检测,并观察海马神经元、神经胶质细胞与微血管内皮细胞超微结构的变化。结果与假手术组比较,不完全脑缺血损伤大鼠全血低切黏度、全血高切黏度、血浆黏度、红细胞刚性指数、红细胞聚集指数明显升高,脑内MDA、NO含量明显升高,SOD活力明显下降,海马神经元、神经胶质细胞与血管超微结构发生明显改变。脑力智宝能显著降低全血高切黏度、血浆黏度、红细胞刚性指数、脑组织内MDA、NO含量,提高SOD活力,并明显改善海马神经元、神经胶质细胞与血管超微结构的损伤。结论脑力智宝对脑缺血损伤具有保护作用,其作用机制可能与其促进血液循环,改善血液流变性,减轻自由基损伤,从而改善脑组织的超微结构有关。

Objective To observe the protective effect of Naolizhibao on incomplete cerebral ischemia in rats. Methods The incomplete cerebral ischemia model in rats was established by ligating the bilateral common carotid arteries. They were divided into the model group, the nimodipine group and the NLZB group, a normal group was set for control. 30 days after operation, the hemorheology, the contents of MDA and NO and the activity of SOD in brain were detected. The uhrastructural changes of neuron, neurogliocyte and microvascular endothelial cell of Hippocampus were observed with transmission electron microscope. Results Compared with normal group, the high and low share rate blood viscosity （ηbh and -ηbl）, plasma viscosity（ηp）, erythrocyte rigidity index（ TK value） , erythrocyte aggregation index （EAI） and the contents of MDA and NO in brain of model rats were significantly increased, while the activity of SOD in brain was lowered. The ultrastructures of neuron, neurogliocyte and microvascular endothelial cell of Hippocampus were damaged severely. After treatment with Naolizhibao, ηbh, ηbh, TK value and the contents of MDA and NO in brain were significantly decreased, the activity of SOD in brain were improved, the injury of Hippocampus were obviously inhibited. Conclusion It is suggested that Naolizhibao has protective effect against cerebral ischemia injury via improving the blood circulation, inhibit free radical production and alleviating the changes of ultrastructures in brain.