促肝细胞生长素对体外培养成人肝细胞增殖和细胞周期的影响

Effects of promoting hepatic growth factor on the proliferation and cell cycle of human hepatocyte in vitro

目的观察促肝细胞生长素(PHGF)在成人肝细胞(HH)增殖和细胞周期中的作用。方法体外培养一定时间后,HH分成空白对照组和5个PHGF剂量(10、20、40、80、160)μg/ml干预组,采用台盼蓝染色,计数CCK-8检测细胞增殖,流式细胞术检测细胞周期。结果PHGF对HH增殖有明显促进作用,PHGF20和40μg/ml两组HH增殖明显大于对照组(P<0.05);与对照组相比,PHGF作用48h后,20和40μg/ml两组G0/G1期细胞比例减少,S期G2/M期细胞比例增加(P<0.05)。结论在一定剂量范围内,PHGF促进HH增生,其可能机制是通过促进DNA合成,增加进入S期细胞数目实现的。

Objective To observe the effects of promoting hepatic growth factor（PHGF） on the proliferation of human hepatocyte（HH） and cell cycle. Methods After cultured in vitro for a certain period of time, the hepatocyter were divided into control group and 5 PHGF intervention groups according different PHGF concentrations of 10,20,40,80 and 160μg/ml. Trypanblue staining was performed for cell proliferation counting by adding CCK-8. Flow cytometry was used for cell cycle testing. Results PHGF had a significantly promoting effect on the proliferation of HH. The proliferation of HH was significantly more in PHGF 20μg/ml or 40μg/ml group than that in the controls（（P〈0.05）. Compaered with the controls, the ratios of G0/G1 phase cells in the groups of 20 μg/ml and 40μg/ml were decreased and the ratio of S phase and G2/M phase ceils increased at 48 hours after PHGF added （P〈0. 05）. Conclusion PHGF can promote the proliferation of HH in the certain concentrations, which may be related to an increased percentage of the cells entering S phase by promoting DNA synthesizing.