登革2型病毒NS1-NS2a基因真核表达载体的构建及鉴定
摘要
目的构建登革2型病毒NS1-NS2a基因真核表达质粒。方法RT-PCR扩增NS1-NS2a基因片段后,将其克隆入真核表达载体pReceiver-M01a;通过PCR、酶切、测序和间接免疫荧光染色鉴定重组质粒。结果经PCR扩增、酶切和测序验证,重组质粒构建正确,命名为pRe-NS1-NS2a;重组质粒转染Vero细胞后,间接免疫荧光染色显示细胞质中有登革2型病毒特异性蛋白的表达。结论成功构建了真核表达质粒pRe-NS1-NS2a。
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2009年第6期557-559,共3页
Journal of Third Military Medical University
基金
国家自然科学基金(30471552,30640065,30671853)
北京市自然科学基金(5082004)~~
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