木瓜微粉及其对胆酸钠和重金属吸附特性研究

In vitro Absorption towards Bile Sodium and Heavy Metal with Chaenomeles sinensis (Thouin) Koehne Fruit Micro-powder

研究了吸附时间对木瓜微粉吸附胆酸钠的影响,并分别对金属混合液浓度、模拟人体胃肠环境的pH条件、木瓜粉的粒径对木瓜微粉吸附混合液中Cu2+、Pb2+、Cd2+、Hg2+的能力进行分析,以明确木瓜微粉及其体外对胆酸钠、Cu2+、Pb2+、Cd2+、Hg2+的吸附特性。结果显示:(1)随粉碎时间的延长,微粉所占的比例增大,粒径分布更加均匀,粉碎过程中存在粉碎-团聚-再粉碎的动态变化。(2)木瓜微粉对胆酸钠的吸附随吸附时间的延长,吸附量显著增加,粗粉和粉碎10、60 min得到的微粉A、C在30 min内对胆酸钠(0.2%)吸附达到了平衡,粉碎30 min得到的微粉B在吸附60 min时对胆酸钠仍有显著的吸附能力。(3)在试验范围内,木瓜微粉B对Cu2+、Pb2+、Cd2+、Hg2+的最适吸附浓度分别为3.00、3.00、1.00、0.01μg/mL。(4)在pH为7时对Cu2+、Pb2+、Cd2+、Hg2+的吸附率分别比pH为2时高出40.85%、21.07%、27.33%、66.38%。(5)不同粒径的木瓜粉对Cu2+的吸附率随粒径的减小而降低,对Hg2+的吸附率则增加,但对Pb2+和Cd2+的吸附率没有显著差异。研究表明,木瓜微粉在体外具有良好的吸附胆酸钠、Cu2+、Pb2+、Cd2+、Hg2+作用,可广泛用于食品和药品中。

The effect of time on absorption capacities towards bile sodium by Chaenomeles sinensis （Thouin） Koehne fruit micro-powders were investigated,and the absorption capacities towards Cu2＋, Pb2＋ ,Cd2＋, Hg2＋ in vitro according to initial metal concentration,pH,and powder particle size were studied. The results showed that：（1）the proportion of micro-powder increased and the particle size distribution more uniformity with the superfine pulverizing time, and the change of particle size was dynamic balance process in- cluding grind-agglomeration-grind. （2）With the increase of absorption time, the absorption capacity of Chaenomeles sinensis （Thouin） Koehne fruit micro-powders towards bile sodium （0.2%） also rose significantly. Within 30 minutes,the absorption towards bile sodium with coarse powder and micro-powder A,C were reached a plateau,while micro-powder B still had significant absorption at 60 minutes. （3）Under the experimental conditions, the optimum absorption concentration of micro-powder B towards metals were Cu2＋ （3.00 μg/mL）, Pb2＋ （3.00 μg/mL）, Cd2＋ （1.00 μg/mL）, Hg2＋ （0.01 μg/mL） respectively. （4）The absorption towards Cue＋ ,Pb2＋ ,Cd2＋ ,Hg2＋ at pH 7 were over by 40.85%,21.07%,27.33%,66.38% than those at pH 2. （5）The particle size change have no significant influence for Pba＋ and Cd2＋- absorption rate, but there has a downward trend for Cu2＋ and an upward trend for Hg2＋ with the particle size reduction. Therefore,the Chaenomeles sinensis （Thouin） Koehne fruit micro-powders could be applied to the food and drug industry widely due to its better absorption capacities towards bile sodium,Cu2＋ ,Pb2＋ ,Cd2＋ , Hg2＋ in vitro.