丝瓜伤流液中抗氧化物质的初步探讨

Preliminary study on antioxidants in bleeding sap from Luffa cylindrica Roem

丝瓜伤流液对DPPH·、·OH等自由基具有一定的清除作用,对由自由基介导的卵黄脂质过氧化和红细胞溶血具有一定的抑制作用。为推测其中抗氧化物质的化学本质,本文检测与分析了丝瓜伤流液的理化性质和常见抗氧化物质在丝瓜伤流液中的含量。结果显示,丝瓜伤流液中的抗氧化物质是蛋白质、维生素、多糖、还原糖以及黄酮类等物质的可能性较小。伤流液干物质中含氮量较大而蛋白含量较低,其紫外与红外光谱特性,以及清除DPPH·的活性物质的极性较大,分子量小于1000Da等的特性提示,有可能该物质与生物碱类物质相关,至于其确切的化学成分还有待进一步的研究。

Bleeding sap from L.cylindrica had a certain scavenging activity on free radicals, such as DPPH · and · OH,and a certain inhibitory effect on lipid peroxidation of yolk and hemolysis of red blood cells, which were induced by free radicals.In order to speculate upon the chemical essence of the antioxidants in bleeding sap of L. cylindrica,the physicochemical properties of this bleeding sap and contents of conventional antioxidants in this bleeding sap were studied. Results demonstrated that the probability of the antioxidants being protein, vitamin, polysaccharide,reducing sugar and flavonoid etc was little. But according to these characters, such as the relatively high nitrogen content and relatively low protein content of this bleeding sap,the polar molecule and low molecular weight of the antioxidant which could scavenging DPPH · etc,maybe the antioxidant in the bleeding sap from L.cylindrica was correlative with alkaloids.As to its precise chemical essence was still need further study.