摇瓶培养条件对红法夫酵母突变菌株UV-801e-S015产虾青素的影响

Effect of culture conditions on the anstaxanthin production of the mutantstrain UV-801e-S015 of Phaffia Rhodozyma in shake flask cultures

利用响应面法研究了培养条件和培养基组成对红法夫酵母突变株UV-801e-S015虾青素产量的影响,结果表明,温度和碳氮源浓度是影响生物量和虾青素产量的主要因素,适合生物量的积累的发酵温度为23℃,而20℃则有利于虾青素的合成,且两者最适培养基的组成也不同。以此确定了以发酵60h为界,将发酵分为两阶段,不同阶段采用不同的培养基和培养温度的发酵方法,实验证明,该方法可有效提高突变株的虾青素产量、生长速率和生物量。

The anstaxanthin production of the mutant strain UV-801e-S015 of Phaffia rhodozyma in shake flask cultures was studied through factorial design and response surface methodology. The results showed that the temperature and the concentrations of C-source and N- source had obvious effects on growth rate and anstaxanthin production of the mutant.The suitable temperature for biomass was 23℃, while that was 20℃ for anstaxanthin production, and the suitable compositions of the medium were different to the two. Finally a fermentation method was found, which divided the fermentation to two stages, the divided line was 60h, and the medium and temperature were different because of the different point.The results showed that the growth rate, anstaxanthin production and biomass of the mutant were enhanced significantly with cultured in this method.