摘要
建立了对二甲氨基苯甲醛分光光度法分析奶粉中L-羟脯氨酸含量的测定方法。奶粉样品经酸水解,释放出的L-羟脯氨酸经氯胺T氧化,与对二甲氨基苯甲醛在一定温度下反应生成红色化合物,在561nm下测定吸光值。该方法的线性范围为0.002~3μg/mL,回收率在90.6%~94.1%,相对标准偏差<1.5%。该方法操作简单、准确度高,可用于测定奶粉中L-羟脯氨酸含量。
The method of P-dimethylaminobenzaldehyde spectrophotometry was established for determination of L-hydroxyproline in milk powder. After hydrolyzed with acid, L-hydroxyproline was released and oxidized by Chloramine T,L-hydroxyproline oxide reacted with P-dimethylaminobenzaldehyde and formed red compounds under certain temperature, the absorbance could be detected at 5dlnm. The liner range of this method was 0.002-3μg/mL, recovery range was 90.6%-94.1% and RSD was less than 1.5%.This method was simple and accurate.It can be used to determine L-hydroxyproline in milk powder.
出处
《食品工业科技》
CAS
CSCD
北大核心
2009年第3期313-314,318,共3页
Science and Technology of Food Industry
关键词
对二甲氨基苯甲醛
L-羟脯氨酸
奶粉
分光光度法
P- dimethylaminobenzaldehyde
L- hydroxyproline
milk powder
spectrophotometry