海马脑片氧糖剥离模型中血晶素对脑红蛋白表达的影响

Neuroprotective of neuroglobin induced by hemin in the hippocampal organotypic tissue cultures after combined oxygen/glucose deprivation

目的：观察血晶素（Hemin）对海马脑片氧糖剥离（Oxygen／glucose deprivation，OGD）模型的保护作用及其对脑红蛋白（Neuroglobin，Ngb）表达的影响。方法：取新生8～10dSD大鼠，制备厚约400μm海马脑片，培养2周后筛取完好无特异荧光着色的脑片，随机分为3组：正常培养对照组（HOTC），单纯缺氧缺糖组（OGD），预加Hemin再缺氧缺糖组（Hemin＋OGD）。除HOTC组外，其余各组在5％CO2、95％N2的混合气体中培养1．5h，其后恢复培养24h。冰冻连续冠状切片，分别作尼氏染色、Ngb的免疫组织化学染色。应用免疫组织化学染色和图像分析处理技术观察海马脑片Ngb蛋白表达，并观察神经元形态学改变。结果：OGD组锥体细胞发生坏死，锥体细胞数量明显减少；Hemin＋OGD组坏死较前者轻，锥体细胞数较前者多。OGD组海马脑片中形态结构正常的阳性细胞少，多数阳性神经元结构模糊、肿胀，Ngb的表达强于HOTC组，二者相比有显著性差异（P〈0．05）；Hemin＋OGD组海马脑片中大部分细胞形态结构正常，少数阳性神经元结构模糊、肿胀，Ngb蛋白的表达较HOTC组和OGD组明显增强（P〈0．01）。结论：血晶素可减轻海马脑片缺糖缺氧性损伤，其机制可能与其诱导神经元表达Ngb密切相关。

Objective： To investigate the neuroprotective role of hemin and its effect on the expression of neuroglobin in the hippocampal organotypic tissue cultures （ HOTC ） after combined oxygen/glucose deprivation （ OGD ）. Methods： SD rats, 8-10-days old, were sacriiced for brain. HOTC were made and randomly divided into three groups： HOTC, HOTC undergoing OGD （OGD）, HOTC undergoing OGD following pretreatment with hemin （Heroin ＋ OGD）. The expression of neuroglobin was detected by immunohisto-chemical technique and computer-assisted image analysis. Results： In OGD group, hippocampal organotypic tissue lost normal structure and neurons were damaged severely. Pretreatment with hemin could effectively reverse the damage of the neurons. The expression of neuroglobin in the Hemin ＋ OGD group were significantly stronger than that in the OGD group. Conclusion： Hemin has protective effect on injury induced by OGD in rat hippaocampal slices, which is associated with enhanced expression of neuroglobin.