DDR1a在结肠癌细胞株中的表达及与基质金属蛋白酶2,9的关系

Expression of DDR1a in colon carcinoma cells and its relationship with matrix metalloproteinase 2,9

目的:探讨结肠癌细胞中盘状结构域受体酪氨酸激酶1(Discoidin domain receptor tyrosine kinases1a,DDR1a)的表达和基质金属蛋白酶2(Matrix metalloproteinase 2,MMP2)、基质金属蛋白酶9(Matrix metalloproteinase9,MMP9)的活性及其相互的关系。方法:用Westernblot检测结肠癌细胞株和人正常结肠平滑肌细胞株中DDR1a表达,明胶酶谱法检测各细胞株中的MMP2,MMP9的活性。结果:侵袭能力强的结肠癌细胞LOVO中,DDR1a呈显著高表达(P<0.01)及MMP2、MMP9呈显著高活性(P<0.01),此外在所测细胞中DDR1a表达与MMP2及MMP9活性呈显著正相关(P<0.05)。结论:本研究提示DDR1a可作为反映结肠癌细胞侵袭力生物学指标之一。

Objective：To investigate the expression of DDRla and the activities of MMP2, MMP9 in colon carcinoma cells and their mutual relations. Methods：The expression of DDRla in colon carcinoma cell and human colon smooth muscle cell was detected by Western blot,the activities of MMP2, MMP9 were examined by gelatin zymography analysis. Results：The expression of DDRla and the activities of MMP2, MMP9 in colon carcinoma cell （LOVO） which has stronger invasive behavior were significant higher （P〈0.01）. There was significant positive correlationship between the expression of DDRla and the activities of MMP2, MMP9 in tested cells （P〈0.05）. Conclusion：DDR 1 a may act as one of biological indicators of the invasion in colon carcinoma ceils.