期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

靶向survivin的siRNA联合顺铂抑制肺腺癌A549细胞增殖的实验研究 被引量：3

Empirical study on the anti-proliferation effect of siRNA against survivin combined with DDP on human lung cancer cell line A549
下载PDF
导出
摘要 目的:研究靶向survivin的siRNA和顺铂联用对肺癌细胞A549的增殖抑制作用。方法:将A549细胞分为空白对照组,阴性对照质粒pSilencer2.0-NC转染组,顺铂处理组,pSilencer2.0-SVV2转染组,pSilencer2.0-SVV2转染+顺铂处理组。转染采用脂质体法。MTT法检测靶向survivin的siRNA和顺铂对A549细胞增殖的抑制作用;RT-PCR法检测A549细胞survivin mRNA转录水平变化情况;流式细胞术和TUNEL法检测A549细胞凋亡情况。结果:MTT法示:pSilencer2.0-SVV2转染+顺铂处理组增殖抑制率为51.38%±1.35%,与其它各组相比抑制率明显增高,具有统计学意义(P<0.05);RT-PCR检测显示空白对照组,阴性对照质粒pSilencer2.0-NC转染组,顺铂处理组survivin mRNA表达无明显变化(P>0.05),pSilencer2.0-SVV2转染组、pSilencer2.0-SVV2转染+顺铂处理组survivin mRNA表达明显下降(P<0.05);流式细胞术和TUNEL法检测pSilencer2.0-SVV2转染+顺铂处理组凋亡率分别为42.48%±2.28%和40.65%±3.53%,与其它各组相比凋亡率明显增高,具有统计学意义(P<0.05)。结论:将靶向survivin的siRNA和顺铂联合应用可以协同抑制A549细胞增殖,共同发挥诱导细胞凋亡作用。 Objective： To investigate the anti-proliferation effect of siRNA against survivin combined with DDP on human lung cancer cell line A549.Methods： A549 cells were divided into five different treatment groups： blank control group, pSilencer2.0-NC group, DDP group,pSilencer2.0-SVV2 group,and pSilencer2.0-SVV2＋DDP group. LipofectamineTM 2000 was used to transfect A549 cell. The effects on cell proliferation and apoptosis were analyzed by MTT assay, flow cytometry, and TUNEL respectively .The expression of survivin mRNA was evaluated by RT-PCR experiment. Results： The MTT assay indicated that the anti-proliferation rate of pSilencer2. 0-SVV2＋DDP group was 51.38% ± 1.35% .The anti-proliferation rate of this group was higher than any other group （P〈0.05）.The experiment of RT-PCR showed that the expression of survivin mRNA in pSilencer2.0-SVV2 group and pSilencer2.0-SVV2＋DDP group were lower than that in other groups（P〈0.05 ）, but its level in blank control group, pSilencer2.0-NC group, DDP group had no marked change （P〉0.05）.Flow cytometry and TUNEL indicated that the apoptosis rates of pSilencer2.0-SVV2＋DDP were 42.48% ± 2.28% and 40.65% ± 3.53% respectively, higher than in any other groups（P〈0.05 ）. Conclusion： siRNA against survivin combined with DDP can enhance the inhibitory effect on lung cancer cell line A549 and induce apoptosis in common.
作者 姚草原 江涛 韩晓黎 李凡
机构地区 重庆医科大学附属第一医院呼吸内科 重庆医科大学附属第一医院普外科
出处 《重庆医科大学学报》 CAS CSCD 北大核心 2009年第2期178-181,共4页 Journal of Chongqing Medical University
关键词 RNA干扰 SURVIVIN基因 肺肿瘤 顺铂 RNA interference Survivin Lung cancer cell DDP
分类号 R734.2 [医药卫生—肿瘤]
  • 相关文献

参考文献9

  • 1Chakravarti A, Noll E, Black PM,et al. Quantitatively determined survivin expression levels are of prognostic value in human glioms[J]. J Clin Oncol, 2002,20 ( 4 ): 1063-1068.
  • 2Muchmore SW,Chen J,Jalob C,et al. Crystal structure and mutagenic analysis of the inhibitor ofapoptosis protein survivin[J]. Mol Cell, 2000,6( 1 ) : 173-182.
  • 3Tamm I,wang Y,Sansvillc E,et al. IAP-family protein survivin inhibits easpase and anticaneer drugs[J]. Cancer Res, 1998,58 ( 23 ) : 5313 -5320.
  • 4Hannon GJ.R NA interference[J]. Nature, 2002,418 ( 6894 ):244-251.
  • 5李伟,陈余清,周继红,夏雪梅,李殿明,高华.Survivin反义寡核苷酸联合顺铂诱导肺癌细胞凋亡的研究[J].中华内科杂志,2006,45(5):414-416. 被引量：3
  • 6刘永全,江涛,冯燕梅.抑制survivin基因表达对肺腺癌A549细胞增殖和凋亡的影响[J].重庆医学,2008,37(4):388-390. 被引量：1
  • 7Von Haefen C,Wieder T,Essmann F,et al. Paclitaxel-induced apoptosis in BJAB cells proceeds via a death receptor-independent, caspases-3/-8-driven mitochondrial amplification loop[J]. Oncogene, 2003,22( 15 ) : 2236-2247.
  • 8Song Z,Yao X,Wu M. Direct interaction between survivin and Smac/DIABLO is essential for the anti-apoptotie activity of survivin during taxol-induced apoptosis[J]. J Biol Chem,2003,278(25):23130-23140.
  • 9Tamm L,Wang Y,Sausvile E,et al. IAP-family protein survivin inhibits caspase activity and apoptosis induced by Fas (CD95). Bax, caspases, and anticancer drugs[J]. Cancer Res, 1998,58(23 ) : 5315-5320.

二级参考文献14

  • 1陈日升,齐连权,于长明,付玲,于婷,陈薇.RNA干涉抑制肺癌细胞DNA甲基转移酶1(DNMT1)的表达[J].军事医学科学院院刊,2005,29(2):127-131. 被引量：6
  • 2Altieri DC, Marchisio PC. Survivin apoptosis: an interloper between cell death and cell proliferation in cancer. Lab Invest, 1999, 79:1327-1333.
  • 3Tanabe U, Yagihashi A, Tsuji N, et al. Expression of survivin mRNA and livin mRNA in non-small-cell lung cancer. Lung Cancer,2004,46:299 -304.
  • 4Kappler M, Bathe M, Barrel F, et al. Knockdown of survivin expression by small interfering RNA reduces the clonogenic survival of human sarcoma cell lines independently of p53. Cancer Gene Ther,2004,11 : 186-193.
  • 5Reddy RM, Tsai WS, Ziauddin MF, et al. Cisplatin enhances apoptosis induced by a tumor-selectlve adenovirus expressing tumor necrosis factor-related apoptosis-inducing ligand.J Thorac Cardiovasc Surg, 2004,128:883-891.
  • 6Ambrosini G, Adida C, Altieri DC, et al. A novel anti-apoptos gene, survivin, expressed in cancer and lymphoma [J]. Nat Med,1997,3(8):917.
  • 7Sarela AI,Verbeke CS, Ramsdale J, et al. Expression of survivin,a novel inhibitor of apoptosis and cell cycle regulatory protein, in pancreatic adenocarcinoma [J]. Br J Cancer,2002,86(6) :886.
  • 8Falleni M, Pellegrini C, Marchetti A, et al. survivin gene expression in early-stage non-small cell lung cancer [J]. J Pathol, 2003,200 (5) : 620.
  • 9Karczmarek-Borowska B, Filip A, Wojcierowski J, et al. survivin antiapoptotic gene expression as a prognostic factor in non-small cell lung cancer: in situ hybridization study[J] . Folia Histochem Cytobiol, 2005, 43(4):237.
  • 10Monzo M ,Rosell R,Felip E,et al. A novel anti-apoptosis gene re-expression of survivin messenger RNA as a prognosis marker in non-small-cell lung canaers[J]. Clin Oncol, 1999,17 (7) : 2100.

共引文献2

同被引文献24

  • 1周彩存,徐妍,苏春霞,邓沁芳,陈晓峰,易祥华.ERCC1在非小细胞肺癌中表达及其临床意义[J].中国癌症杂志,2006,16(8):622-625. 被引量：23
  • 2Luu H H,Song W X,Luo X,et al.Distinct roles ol bone morphoge netic proteins in osteogenic differentiation of mesenchymal stem cells [J]. J Orthop Res, 2007,25(5 ) : 665-677.
  • 3Lopez-Coviella I, Mellott T M, Kovacheva V P, et al.Developmental pattern of expression of BMP receptors and Smads and activation of Smadl and Smad5 by BMP9 in mouse basal forebrain[J]. Brain Res, 2006, 1088 ( 1 ) : 49-56.
  • 4Lopez-Coviella I, Follettie M T, Mellott T J, et al. Bone morphogenetic protein 9 induces the transcriptome of basal forebrain cholinergic neurons [J]. Proc Natl Acad Sci, 2005, 102(19) : 6984-6989.
  • 5David L,Mallet C,Keramidas M,et al.Bone morphogenetic protein -9 is a circulating vascular quiescence factor[J]. Circ Res, 2008,102( 8 ) : 914-922.
  • 6Scharpfenecker M, van Dinther M, Liu Z, et al. BMP-9 signals via ALK1 and inhibits bFGF-induced endothelial cell proliferation and VEGF-stimulated angiogenesis[J]. J Cell Sci., 2007, 120(6 ) : 964-972.
  • 7Ye L, Kynaston H, Jiang W G., et al. Bone morphogenetic protein-9 induces apoptosis in prostate cancer cells, the role of prostate apoptosis response-4[J]. Mol Cancer Res, 2008, 6(10) : 1594-1606.
  • 8Herrera B, Inman G J.A rapid and sensitive bioassay for the simult aneous measurement of multiple bone morphogenetic proteins. Identifi cation and quantification of BMP4, BMP6 and BMP9 in bovine and human serum [J]. BMC Cell Biol, 2009,10(20): 1-11.
  • 9Li F,Ling X.Survivin study:an update of "what is the next wave" [J].J Cell Physiol,2006,208 (3):476-486.
  • 10Ambrosini G,Adida C.A novel anti-apoptosis gene, survivin,expressed in cancer and lymphoma [J]. Nature Med,1997,3(8):917-923.

引证文献3

二级引证文献2

重庆医科大学学报
2009年 第2期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部