钙结合蛋白S100A7在肺癌中的选择性表达

Selective Expression of Calcium-Binding Protein S100A7 in Lung Cancer

背景与目的S100A7是S100蛋白家族成员,它的过表达与一些癌的发生和转移密切相关,本文旨在探讨S100A7在肺癌中的差异表达。方法采用RT-PCR、Western blot和免疫组化等技术探讨了S100A7蛋白在肺癌及其癌旁组织中的表达差异。结果S100A7只在肺鳞癌和大细胞肺癌组织中有表达,而在肺腺癌、小细胞肺癌及其他亚型的肺癌组织、癌旁组织和正常肺组织中不表达;并发现S100A7蛋白的表达在高分化的癌细胞中较强。结论S100A7蛋白可选择性地在不同亚型的肺癌中表达,很可能成为肺癌亚型诊断的标志蛋白之一。

Background and objective S100A7 is a member of S100 family and its overexpression has been previously associated with carcinogenesis of certain cancers. The aim of this study is to explore the expression of calcium-binding protein S100A7 in lung cancer. Methods In the present study, RT-PCR, Western-blot and immuno- histoehemistry were used to analyze the different expression of $100A7 in lung cancer tissues and paired noncancerous lung tissues. Results The specific expression of S100A7 was found in squamous cell carcinomas and large cell lung carcinomas, whereas it was not detected in adenoeareinomas, small cell carcinomas, other cell types of lung cancer and normal lung tissues as well as paired noncancerous lung tissues. Conclusion S100A7 was selectively expressed in squamous cell carcinomas and large cell lung carcinomas and may serve as a potential marker for lung cancer diagnosis.