VEGF-AsiRNA对人肝癌HepG2细胞体外增殖、侵袭及化疗敏感性的影响

Effects of VEGF-A siRNA on the Proliferation,Invasion and Chemosensitivity of HepG2 Cells

目的本研究应用RNA干扰技术下调人肝癌细胞株HepG2中VEGF-A的表达,观察该细胞的体外增殖、侵袭能力,进一步研究调节VEGF-A的表达对肿瘤细胞药敏性的影响。方法采用RT-PCR、Western Blot分析干扰前后HepG2细胞中VEGF-A的表达情况;体外侵袭实验检测干扰前后HepG2细胞的侵袭能力;MTT法分析干扰前后HepG2细胞的增殖情况及该细胞的药敏性。结果HepG2/RNAi细胞中VEGF-A的表达与未给予RNA干扰处理的对照组及RNA干扰对照组相比出现明显下调;该细胞的增殖及穿过基质胶的侵袭能力下降;同时上调该细胞对化疗药物的敏感性。结论人肝癌细胞中VEGF-A的表达与肿瘤细胞的增殖、侵袭及对抗肿瘤药物的敏感性密切相关,为肿瘤的化学治疗提供新靶点。

Objective To investigate the possible effects of VEGF-A on the proliferation, invasion and chemosensitivity of human hepatocarcinoma cell line HepG2, we used an RNA interference （RNAi） approach to silence VEGF-A expression. Methods The expression level of VEGF-A of HepG2/siRNA ceils was checked by RT-PCR and Western Blot. The inhibitory effect of RNAi on VEGF-A ceils invasion in vitro was demonstrated by ECM invasion assay. The in vitro proliferative ability and chemosensitivity of VEGF-A deficient cells were determined by MTT assay. Results The expression of VEGF-A was significantly reduced in VEGF-A/siRNA cells after 30h transfection, compared with both the RNAi control and the VEGF-A cells. The reduced VEGF-A expression also attenuated the proliferative, invasive ability, as well as increased chemosensitivity in HepG2/siRNA cells. Conclusions Our current results indicate that the expression of VEGF-A functionally mediates proliferation, invasion and chemosensitivity of tumor cells and is a potential target for therapeutic anti-cancer drugs.