胃复康对人SGC-7901胃癌细胞株p53、bcl-2基因表达的影响

The Alteration of Expression of p53 and bcl-2 by Wei-Fu-Kang in the Human SGC-7901 Stomach Cancer Cells

目的探讨中药胃复康对人SGC-7901胃癌细胞株p53、bcl-2基因表达的影响。方法PRMI1640血清培养的人SGC-7901胃癌细胞株分为胃复康实验组、生理盐水对照组和顺铂(DDP)化疗组。利用血清药理学方法制备药物血清,作用48h后,应用免疫细胞化学方法和图像分析方法观察胃复康、DDP对人SGC-7901胃癌细胞株p53、bcl-2基因表达影响。结果免疫细胞化学法显示抑癌基因p53和癌基因bcl-2主要定位在细胞质内。胃复康、DDP药物血清作用后,p53表达明显增强,bcl-2表达明显降低;随着作用时间延长,两种基因表达改变更明显。胃复康、DDP药物血清组p53和bcl-2表达的光密度值(AOD)与对照组差异显著(P<0.01)。结论中药胃复康药物血清上调p53和下调bcl-2的表达可能是其抑制胃癌细胞株SGC-7901的作用机制之一。

Objective To investigate the alteration of expression of p53 and bcl-2 by Wci-Fu-Kang in the human SGC-7901 stomach cancer cells. Methods Human SGC-7901 stomach cancer cells cultured in PRMlI640 were divided into 3 groups：wei-fu-kang treating group, saline control group and cisplatin chemiotherapy（DDP） group. 48 hours later, immunohistochemical techniques and microimage analysis system were used to observe and analyze the expression of p53 and bcl-2. Results The positive p53 and bcl-2 products were localized in the cytoplasm.The expression of p53 was higher, expression of bcl-2 was lower in wei-Fu-Kang treating group and DDP group than in saline control group（P〈0.01）, with the time dependence. Conclusion Upregulation of p53 and downregulation of bcl-2 by Wei-Fu-Kang might be one of the mechanisms of the inhibitory function of chinese herb Wei-Fu-Kang on SGC-7901 stomach cancer cells.