DLL4、VEGF在肺腺癌中的表达及其与肿瘤血管生成的关系

Expression of DLL4 and VEGF in Lung Adenocarcinoma and their Relationship with Angiogenesis in Tumor

背景与目的血管发生(angiogenesis)依赖于多种促进血管发生因子和抑制血管发生因子的综合交互作用调控。血管内皮生长因子(VEGF)和Notch信号传递途径(Notch signaling pathway)参与了此过程。本研究旨在探讨肺腺癌组织中Notch配体DLL4、VEGF的表达及其与肺腺癌血管生成的关系及临床意义。方法应用免疫组织化学方法检测80例肺腺癌(包括细支气管肺泡癌和普通肺腺癌)石蜡切片组织中DLL4、VEGF和CD34的表达。结果DLL4、VEGF的表达与肺腺癌患者的肿瘤直径、临床分期、组织学分级、淋巴结转移密切相关,DLL4阳性表达病例中VEGF表达率明显比DLL4阴性表达病例高,DLL4、VEGF共表达时与微血管密度的相关性比DLL4单独表达更明显。DLL4的表达在普通肺腺癌中明显高于细支气管肺泡癌。结论肺腺癌的预后与血管生成明显相关,DLL4的高表达与肺腺癌转移预后密切相关。

Background and objective Angiogenesis depends on the interaction of a variety of promoting factors and inhibiting factors. Vascular endothelial growth factor （VEGF） and Notch signaling pathway take part in this process. This experiment investigates the expression of Notch ligand DLL4 and VEGF in lung adenocarcinoma and their relationship with angiogenesis in tumor. Methods Immunohistochemieal method was used to detect DLL4, VEGF and CD34 protein expression in 80 cases of lung adenoearcinoma （including bronchioloalveolar carcinoma and common lung adenocarcinoma） paraffin section tissues. Results The expression of DLL4 and VEGF was closely related to tumor diameter, clinical stage, histological grade and lymph node metastasis, the VEGF expression rate in DLL4 positive expression cases was significantly more than the DLL4 negative cases, the correlation between mierovascular density and DLL4, VEGF co-expression was more significant, the expression of DLL4 in common lung adenocarcinoma was significantly higher than that in bronchioloalveolar carcinoma. Conclusion The prognosis of lung adenocareinoma is significant correlated with the angiogenesis, high expression of DLL4 is closely related to the metastasis and the prognosis.