口腔癌相关成纤维细胞的体外生物学行为研究

In vitro biological behavior of oral carcinoma associated fibroblasts

目的探讨口腔鳞癌中癌相关成纤维细胞(carcinoma associated fibroblasts,CAFs)的体外生物学行为。方法组织块贴壁法培养CAFs及牙龈正常成纤维细胞(normal fibrolasts,NFs);通过形态观察,免疫组化染色,细胞增殖活性、贴壁率、迁移能力等测定,比较两种细胞的生物学行为差异。结果与NFs相比,CAFs特征性表达成纤维激活蛋白(fibroblast activation protein,FAP)、α-平滑肌肌动蛋白(α-smooth muscle actin,α-SMA)及基质金属蛋白酶-2(matrix metalloprotease-2,MMP-2),具有更高的增殖活性、贴壁率及迁移能力(P<0.05)。结论口腔癌CAFs特征性表达相关蛋白,体外增殖、粘附、迁移能力均高于NFs。

Objective To investigate the in vitro biological behavior of carcinoma associated fibroblasts（CAFs）. Methods Primary cultured CAFs and normal fibroblasts（NFs） in vitro were prepared. Related research was conducted on the biological disparity between the two strains through immunohistochemistrical staining, proliferative activity detection and migration assay. Results Evident disparity of proliferative activity and migration length was discovered between CAFs and NFs（ P 〈 0.05）. CAFs characteristically expressed FAP, MMP-2, α-SMA. Conclusions The ability of generation, invasion, adhesion of CAFs exceeded NFs in vitro and characteristically expressed specified protein.