miRNA体内作用靶标鉴定的策略

Strategies to Identify miRNA in vivo Targets

miRNAs是一类重要的基因表达调节因子,近年的研究表明miRNA在控制细胞的生长发育、分化、凋亡等过程中发挥着十分重要的作用。但对miRNA作用机制和分子功能的研究却进展缓慢,归其原因是miRNA和靶标之间非完全配对,缺乏快捷有效的靶标鉴定方法。因此,就miRNA靶标鉴定的策略作一综述。miRNA调节靶标的鉴定有助于揭示一些疾病的致病机理,发现可用于治疗的新分子靶标,为基因治疗奠定基础。

MiRNAs are an important class of gene regulators, and miRNAs play a pivotal role in the regulation of genes involved in diverse processes such as development, differentiation, and cellular growth control. But little is known about it＇ s role and molecular mechanisms due to lack of efficient and effective target identification methods. Therefore, recent progress on the adjustment target of miRNA was reviewed. The adjustment target of miRNA is very helpful to reveal the pathogenetic of some diseases, discover novel molecular targets for treatment and establish foundation for gene therapy.