摘要
研究了黄芩及其水煎废渣中黄芩苷的提取工艺,并比较了两种提取物中黄芩苷的含量和抗氧化活性.通过正交试验得到的黄芩中黄芩苷的水煎煮法提取工艺为:提取3次,料液比1∶15,每次提取1.5 h;水煎煮黄芩废渣中黄芩苷的醇法超声强化提取工艺为:乙醇体积分数70%,料液比1∶15,超声功率150 W,提取时间30 min;从黄芩及其水煎废渣中提取黄芩苷粗品的得率分别为4.57%和7.78%,但两者黄芩苷含量和抗氧化活性相近.
A lot of bio-active ingredients remains in the wastes of Chinese herbal medicines decocted by water, which can be reused as a resource. This paper investigated the optimum conditions of extracting baicalin by decocting baikal skullcap root （BSR） firstly and then pre- cipitating baicalin with acid, the optimum technical conditions of extracting baicalin in decocted BSR wastes by combining use of alcohol and ultrasound, the baicalin content in 2 extracts by HPLC, and the anti-oxidation activity of the 2 extracts. As a result, the optimum conditions of extracting baicalin by decocting BSR are as follows： the ratio of feed to liquid 1 ： 10, extracting time 1.5 h, and extracting times 3. The optimum technical conditions of extracting baicalin from decocted BSR residue are. the ethanol volume fraction 70%, the ratio of feed to liquid 1 ： 15, power 150 W, and extraction time 30 min. The yield of raw baicalin from BSR and its decocted residue are 4. 57% （w/w） and 7. 78% （w/w） respectively, and the 2 extracts have close baicalin content and anti-oxidation activity.
出处
《陕西科技大学学报（自然科学版）》
2009年第1期74-77,共4页
Journal of Shaanxi University of Science & Technology
基金
广东省科技计划项目(2007B080401010)
关键词
黄芩
水煎黄芩废渣
黄芩苷
提取
抗氧化
baikal skullcap root （BSR）
decocted BSR residue
baicalin
extraction
anti-oxidation
