RP-HPLC法测定不同产地枳实中的柚皮苷、橙皮苷及新橙皮苷

Determination of Naringin,Hesperidin and Neohesperidin Content in Frutus Aurantii Immaturus from Different Places by RP-HPLC Approach

目的：建立同时测定枳实中柚皮苷、橙皮苷和新橙皮苷含量的方法。方法：采用反相高效液相色谱法分离检测柚皮苷、橙皮苷和新橙皮苷，色谱柱为HypersilODS2（250mm×4．6mm，5μm大连依利特公司），流动相为乙腈-水（20：80），流速为1．0mL／min，检测波长283nm，柱温为30℃。结果：柚皮苷、新橙皮苷在1．00～7．50μg，橙皮苷在0．40～3．00μg与峰面积呈良好的线性关系。柚皮苷、橙皮苷、新橙皮苷的平均回收率分别为100．56％（RSD：0．55％）、100．20％（RSD=1．00％）、101．14％（RSD=1．74％）。结论：该方法简便、准确、可靠、重复性好，可用于枳实药材的质量控制。

A method was established to determine the contents of Naringin, Hesperidin and Neogesperidin in Frutus aurantii immaturus. Reversed-phase HPLC was used to find the contents of Naringin, Hesperidin and Neogesperidin in Frutus aurantii immaturus. The chromatographic conditions were as follows： Hyspersil ODS2 （250 mm × 4.6 mm,5 μm elite, Dalian）, mobile phase of acetoni- trile-water （20： 80）, flow rate： 1.0 mL/min, detection wavelength： 283 nm and the column temperature： 30 ℃. The calibration curves were linear in the range of 1.00 - 7.50 μg for Naringin and Neohesperidin, and 0. 40 - 3.00 μg for Hesperidin. The average recovery of Naringin Hesperidin and Neogesperidin were 100. 56% （ RSD =0.55% ）, 100. 20% （ RSD = 1.00% ）, 101.14% （ RSD = 1.74% ） respectively. This method is simple, accurate, reliable and has good reproducibility. It can be used for the quality control for the production of medicine containing Frutus anrantii immaturus.