树突状细胞在鼻息肉组织中的浸润及其意义

Expression and distribution of dendritic cells in nasal polyps

目的观察树突状细胞(DC)在鼻息肉组织中的分布特点,探讨DC在鼻息肉发病机制中的作用。方法选择手术摘除鼻息肉30例、正常下鼻甲黏膜10例,采用免疫组织化学方法检测鼻息肉组织中S-100蛋白表达情况,免疫荧光双标记(CD1a/CD40)及低照度荧光显微镜图像分析检测方法,观察DC在鼻息肉组织及正常下鼻甲黏膜中的分布情况,并初步探讨鼻息肉组织中DC与T淋巴细胞之间的关系。结果鼻息肉组织中S-100阳性细胞及CD1a/CD40双阳性细胞数量较下鼻甲黏膜明显增多,其差异有显著统计学意义(P<0.01);鼻息肉组织与下鼻甲黏膜中DC的密度分别为(7125±2575)个/cm2和(589±456)个/cm2,两者的差异有显著统计学意义(P<0.01)。此外,鼻息肉组织中DC主要分布在黏膜下层,有从外向内递减的趋势,并且DC表面CD40分子为阳性。结论鼻息肉组织中DC浸润显著,DC可通过CD40/CD40L共刺激因子与T细胞产生相互作用,在鼻息肉发病及疾病发展过程中发挥重要作用。进一步探讨DC在鼻息肉发病机制中的作用,将会拓宽鼻息肉的研究视野,为鼻息肉的防治提供新的干预靶点。

Objective To investigate the expression, distribution and function of dendritic cells （DC）, and study the role of the DC in the nasal polyps. Methods Our study involved 40 participants,30 participants with nasal polyps in study group and 10 participants with normal inferior turbinates in control group. We used double immunostaining method to check the CD1a/CD40 and the images were analyzed with Axioplan 2 microcopy. Results （ 1 ） There were much more double-stained cells （DC） in nasal polyps than in normal inferior turbinates, and the difference was distinct significant （ P 〈 0.01 ） ; （ 2 ） The distribution areas, number and density of double-stained cells in nasal polyps were greater than in normal inferior turbinates（P 〈 0.01 ）;（ 3 ）The double-stained cells （DC） were mainly in the lamina propria below the mucous membrane. Conclusions Our study demonstrate that dendritic cells involved in pathogenesis of nasal polyps and there were some CD40 positive DCs suggest that it were related to the a reciprocity relationship of DCs and T lymphocytes.