禾本科粒毛盘菌多糖提取及其抗氧化活性研究

Study on extraction technology and antioxidative activity of polysaccharides produced by Lachnum hyalopus.

对禾本科粒毛盘菌(Lachnumhyalopus)胞内多糖的微波提取工艺进行研究,并研究该多糖对超氧阴离子自由基()和1,1-二苯基苦基苯肼(DPPH)自由基的清除作用.结果表明,粒毛盘菌胞内多糖的最佳提取工艺条件为:微波功率150W,时间3min,液料比80,此条件下多糖得率可达12.05%.粒毛盘菌多糖对和DPPH自由基均有较好的清除作用,多糖浓度为100mg·L-1时,对的清除率达到86.67%;浓度为10mg·L-1时,对DPPH自由基的清除率达到10.79%.表明粒毛盘菌多糖具有明显的抗氧化活性.

Extraction of polysaccharide from Lachnum hyalopus with microwave and its scavenging capacity of superoxide anion radical and 1,1-diphenyl-2-picrylhydrazyl （DPPH） free radical were studied. The results indicate that the optimum conditions for extraction of polysaccharide were as follows： microwave power was 150 W with ratio of liquid to material at 80 and 3 rain of extraction time. Under above conditions, the polysaccharide yield was up to 12.05%. Meanwhile, the scavenging effects of the polysaccharide on O2^- and DPPH free radicals were both significant, which the scavenging effects on the O2^- radical reached 86.67% with 100 mg· L^-1 polysaccharide, and the clearance rate of DPPH free radical reached 10.79 % with 10 mg· L^-1 polysaccharide. So it can be concluded that the polysaccharide displayed obvious antioxidant activities.