响应面优化棉子饼粕中棉酚的提取条件

Optimization of Extraction Technology Condition of Gossypol from Cottonseed Meals Using Response Surface Methodology

采用响应曲面法的Box-Behnken模式,以盐酸和80%乙醇混合溶液作为提取液浸提棉子饼粕中棉酚工艺条件进行了研究,并建立提取棉酚的二次多项数学模型,探讨了主要因素的影响效应及其交互作用。采用响应曲面法优化得出的最佳工艺条件为:浸提温度60℃,提取液用量为26 mL.g-1,盐酸浓度为1 mol.L-1,浸提时间为3.6 h,提取次数1次时,提取量为8.80 mg.g-1。

Optimizing extraction technology of gossypol from acid and 80%ethanol mixed solution by Response Surface cottonseed meals with the hydrochloric Methodology＇s Box-Behnken mode was studied. A quadratic equations model for gossypol extraction was built and effect of main factors and their corresponding relationships were obtained. The optimal extraction conditions of gossypol from cottonseed meals as follows： extraction temperature 60℃, volume of extraetant 26 mL ·g^-1,hydrochlorie acid＇s concentration 1 mol · L^-1, extraction time 3.6 h, extracting times was 1, the yield of gossypol from cottonseed meals by hydrochloric acid and 80% ethanol mixed solution was 8.80 mg ·g^-1