摘要
目的:建立以高效毛细管电泳(HPCE)法鉴别南葶苈子和车前子的方法。方法:采用石英毛细管(60cm×75μm),运行缓冲液为30mmol·L-1磷酸二氢钠(pH8.2),高压进样5s,分离电压为12kV,检测波长为330nm。结果:南葶苈子和车前子的HPCE色谱存在较大差异,容易区分。结论:本法简单、快捷、灵敏,可用于鉴别南葶苈子和车前子。
OBJECTIVE: To identify Descurainia sophia and Semen Plantaginis by high performance capillary electrophoresis (HPCE) . METHODS: HPCE was conducted with a fused silica capillary tube(60 cm×75 μm) taking 30 mmol · L^-1 sodium dihydrogen phosphate as running buffer (pH 8.2). The sample injection was performed by high pressure for 5 s at a separation voltage of 12 kV, an UV detection wavelength of 330 nm. RESULTS: The differences of HPCE eleetrophoregrams between Descurainia sophia and Semen Plantaginis were significant and easily to be differentiated. CONCLUSION: This method is simple, rapid and sensitive, and it can be applied to identify Descurainia sophia and Semen Plantaginis.
出处
《中国药房》
CAS
CSCD
北大核心
2009年第9期683-684,共2页
China Pharmacy
基金
广东省医学科研基金立项课题(A2008398)
南方医院院长基金资助课题(20060019)