HPCE法鉴别南葶苈子和车前子

Identification of Descurainia sophia and Semen Plantaginis by HPCE

目的:建立以高效毛细管电泳(HPCE)法鉴别南葶苈子和车前子的方法。方法:采用石英毛细管(60cm×75μm),运行缓冲液为30mmol·L-1磷酸二氢钠(pH8.2),高压进样5s,分离电压为12kV,检测波长为330nm。结果:南葶苈子和车前子的HPCE色谱存在较大差异,容易区分。结论:本法简单、快捷、灵敏,可用于鉴别南葶苈子和车前子。

OBJECTIVE： To identify Descurainia sophia and Semen Plantaginis by high performance capillary electrophoresis （HPCE） . METHODS： HPCE was conducted with a fused silica capillary tube（60 cm×75 μm） taking 30 mmol · L^-1 sodium dihydrogen phosphate as running buffer （pH 8.2）. The sample injection was performed by high pressure for 5 s at a separation voltage of 12 kV, an UV detection wavelength of 330 nm. RESULTS： The differences of HPCE eleetrophoregrams between Descurainia sophia and Semen Plantaginis were significant and easily to be differentiated. CONCLUSION： This method is simple, rapid and sensitive, and it can be applied to identify Descurainia sophia and Semen Plantaginis.