HPLC法测定虎杖中大黄素-8-O-β-D-葡萄糖苷的含量

Determination of Physcion-8-O-β-D-glucoside in Polygonum cuspidatum by HPLC

目的:建立以高效液相色谱法测定虎杖中大黄素-8-O-β-D-葡萄糖苷含量的方法。方法:采用D101大孔吸附树脂对虎杖样品进行预处理;色谱柱为Kromasil C18(250mm×4.6mm,5μm),柱温为40℃,流动相为乙腈-水(40∶60),流速为1.0mL·min-1,检测波长为420nm。结果:大黄素-8-O-β-D-葡萄糖苷的检测浓度在5～200μg·mL-1范围内与峰面积积分值呈良好的线性关系;平均回收率为98.63%,RSD=0.99%(n=6)。结论:本方法简便、快速、准确,可用于虎杖药材的质量控制。

OBJECTIVE： To determine the concentration of emodin-8-O-β-D- glucoside in Polygonum cuspidatumby HPLC. METHODS： The giant knotweed rhizome sample was pretreated by D101 macroporous adsorptive resins. The separation of sample was performed on Kromasil C18（250 mm ×4.6 mm, 5μm） with column temperature set at 40 ℃ The mobile phase consisted of acetonitrile water （40 ： 60） at a flow rate of 1.0 mL· min^-1 and a detection wavelength of 420 nm. RESULTS： The linear range of emodin -8-O-β-D-glucoside was 5--200μg·mL^-1 with an average recovery of 98.63% （RSD -0.99%, n =6）. CONCLUSION： The method applied in the study is simple, rapid, accurate, and suitable for the quality control of determination of emodin -8-O-β-D- glucoside in Polygonum cuspidatum . KEY WORDS Emodin -8-O-β-D-glucoside; Polygonum cuspidatum ; HPLC; Macroporous adsorptive resins; Content determination