扶正解毒含药血清对硫酸镍诱导人支气管上皮细胞转化的抑制作用

Inhibitory Effect of Fuzhengjiedu-containing Serum on the Transformation of Human Bronchial Epithelial Cells Induced by Nickel Sulfate

目的观察中药复方扶正解毒含药血清(FJD)对硫酸镍(NiSO4)诱导培养人支气管上皮(16HBE)细胞恶性转化的抑制作用。方法NiSO4多次处理16HBE细胞,利用刀豆凝集素A(ConA)凝集实验和软琼脂集落形成实验进行转化细胞的恶性鉴定;同时添加不同剂量的FJD,观察其抗转化效应。结果NiSO4(400μmol/L)染毒8次(20代)时,细胞生长速度加快,排列紊乱,失去接触抑制,出现复层生长,可被ConA凝集,并可在软琼脂上生长。3种剂量FJD与NiSO4共处理组细胞转化率分别为0.82%、0.47%和0.19%,明显低于单用NiSO4组(4.13%),且呈剂量-效应关系(r=-0.884,P<0.01),细胞ConA凝集反应阴性,不能在软琼脂上生长。结论体外实验显示,FJD具有抑制NiSO4诱导16HBE细胞恶性转化的作用。

Objective To establish a malignant transformation model for human bronchial epithelial cell line（16HBE） with nickel sulfate（NiSO4 ） and observe the effect of Fuzhengjiedu-containing Serum（FJD） on the transformation of 16HBE cells induced by NiSO4. Methods 16HBE cells were treated repeatedly with NiSO4 （400μmol/L） in vitro. Different doses of FJD were added into the media containing NiSO4 , respectively. The identification of malignancy of the transformed cells was carried out by the tests of Con A and the growth on soft agar culture. Results After being treated with NiSO4 for 8 times, cells proliferated rapidly exhibited in extensive random orientation and crossover growth and could be agglutinated by Con A and could grow in soft agar. The transformation frequencies in groups treated with both FJD and NiSO4 were 0. 82%, 0.47% and 0.19% showing significant decrease （P〈0.05～0.01） in dose-dependent manner, as compared with that in the group treated with NiSO4 alone （4.13%）. Cells co-treated with FJD and NiSO4 could not be agglutinated by Con A and could not grow in soft agar. Conclusions In vitro experiments indicate that FJD shows anti-transforming activity in human bronchial epithelial cell line exposed to NiSO4.