钙调素拮抗剂对荷瘤小鼠主要器官钙调素(CaM)水平的影响

EFFECT OF CALMODULIN ANTAGONIST ON CALMODULIN CONTENT IN ORGANS OF TUMOR BEARING MICE

本文采用磷酸二酯酶（PDE）法，测定了小檗胺衍生物EBB对荷S180瘤小鼠的主要器官和瘤体CaM含量的影响．实验结果表明，EBB具有使荷瘤后小鼠脑、肺、肝、脾、肾五个主要器官CaM水平由不正常趋向正常的能力，而已知抗肿瘤药物丝裂霉素和环磷酰胺单独使用时此种能力较弱，但这两种抗肿瘤药物与EBB联合使用时，能使荷瘤鼠诸器官的CaM水平趋向正常．结果提示，专一性强的CaM拮抗剂在抑制肿瘤的同时可能还具有维护主要器官细胞正常生理功能的作用．

The effect of Calmodulin(CaM) antagonist-EBB on CaM content was measured with PDE assay in major organs and tumor of S180 bearing mice.As compared with normal mice, the experimental results showed the CaM content in five organs (brain, lung, liver, spleen and kidney) was abnormal after tumor bearing. It is EBB other than MMC or CPA that can chage this abnormality, especially soluble CaM content. The CaM content in tumor of therapeutic groups are lower than the control. EBB also can change the abnormality of total protein in organs by MMC, but it can not change the abnormality by CPA.The results suggest that the reason of tumor inhibition by CaM antagonist-EBB may be involved with function that it can counter the CaM content in major organs of S180bearing mice. So it is worthy further to be studied.