大鼠脊髓损伤后适度硬膜外低温的神经保护作用及机制探讨

Neuroprotective effect and mechanism of moderate epidural hypothermia in rats with spinal cord injury

目的探讨适度硬膜外低温(MEH)在大鼠脊髓损伤(SCI)后的神经保护作用及机制。方法选取16只大鼠建立SCI(T9节段)模型,随机分为观察组与对照组各8只,对照组不予任何处理,观察组设计MEH(30℃、48h)系统进行干预,7d后处死大鼠,行运动功能评分和斜面测试,HE染色及双重染色(TUNEL法和抗CC1),检测少突胶质细胞的凋亡及对小胶质细胞的抗炎作用。结果观察组运动功能显著改善,神经元和少突神经胶质细胞的细胞凋亡显著减少,小胶质细胞的活化显著抑制,与对照组相比,P均<0.05。结论MEH在SCI中具有显著的神经保护作用,其机制可能是抑制少突胶质细胞凋亡和抗炎作用。

Objective To investigate the neuroprotective effect and mechanism of moderate epidural hypothermia （ MEH ） on spinal cord injury （ SCI ） in rats. Methods SCI model（T-9 ） was produced in all 16 rats, and then randomly divided into two groups. The control group was treated with no special measure, and the hypothermia group were interfered in MEH system（30 ℃, 48 h）. On the seventh postoperative day all rats were killed, motor function scores, inclined plane test and double stained （TUNEL stain and anti-CCl immune stain）were used to evaluate the apoptosis of oligodendrecytes and the anti-inflammatory effects of microglia. Results Compared with the control group Motor function in the hypothermia group improved, the apoptosis of neurons reduced, the activation of the microglia inhibited （P 〈 0. 05）. Conclusions MEH have significant neuroprotective effect to SCI, its mechanism maybe inhibiting the apoptosis of the oligodendrocytes and the anti-inflammatory effects.