摘要
为满足城市绿化需要，研究了常春藤微繁的培养基和培养程序。ＭＳ培养基中除附加ＢＡ、ＮＡＡ外，添加ＧＡ３，能促进芽的增殖和生长。本文主要讨论了ＧＡ３的作用和较适宜的浓度。常春藤茎尖、茎段培养较适宜的培养基为ＭＳ＋ＢＡ４．０ｍｇ／Ｌ＋ＮＡＡ０．２ｍｇ／Ｌ＋ＧＡ３１．０～２．０ｍｇ／Ｌ。生根培养基为１／２ＭＳ＋ＮＡＡ０．２ｍｇ／Ｌ。
To satisfy the need of city greening ,the tissue cultural media and process for Hedera nipalensis var.sinensis were studied. Apart from BA and NAA, GA 3added to MS medium could promote the multiplication of bud and growth. The study show that the suitable media for shoot tip and stem culture is MS +BA4 0mg/L+NAA0 2mg/L+GA 31 0～2 0mg/L ,and 1/2MS +NAA0 2mg/L for culture of the plant.
出处
《河北林果研究》
1998年第1期86-90,共5页
Hebei Journal of Forestry and Orchard Research
关键词
常春藤
离体培养
植株再生
GA3
观叶植物
Hedera nipalensis var.sinensis
culture in vitro
regeneration of plantlet
GA 3
